Alexa Fluor™ 647 Phalloidin

来自 : 发布时间：2024-05-05

Get the sharpest, brightest images. Try new Invitrogen SlowFade Glass antifade RIMS mountant with refractive index of 1.52. Learn more Alexa Fluor 647 phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, far-red fluorescent Alexa Fluor 647 dye. Selectively stains F-actin Outstanding fluorescence performance Excitation/Emission: 650/668 nm Superior to antibody staining Optimal for fixed and permeabilized samplesGet Superior Results in Actin Staining StudiesPhalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides \'death cap\' mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while Alexa Fluor 647 provides far-red fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluor 647 phalloidin allows high-contrast discrimination of actin staining.Use in Multiple ApplicationsAlexa Fluor 647 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor 647 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and other Alexa Fluor conjugates including secondary antibodies.For research use only. Not intended for human or animal therapeutic or diagnostic use.Related Links Read about labeling F-Actin with phallotoxins. See the full line of Alexa Fluor Dye products. Create a Cellular Masterpiece with Alexa Fluor Phalloidin using the Cell Paint Tool. HUVEC mounted with Prolong Glass and imaged with a EVOS FL 2Human umbilical vein endothelium cells (HUVECs) were fixed and permeabilized. Tubulin (green) was probed with Mouse Anti-beta-3 Tubulin Antibody (Cat. No.  322600) and Alexa Fluor 488 Goat Anti-Mouse Secondary Antibody (Cat. No. A-11029). Actin (red) was stained with Alexa Fluor 647 Phalloidin (Cat. No. A22287) and nuclei (blue) labeled with NucBlue Fixed Cell ReadyProbes Reagent (Cat. No. R37606). Cells were mounted in ProLong Glass Antifade Mounting Medium (Cat. No. P36980) and slides were stored in freezer (moisture free) for three months. Cells were imaged on EVOS FL Auto 2.0.\" data-omni-action=\"View figure\">

HUVEC mounted with Prolong Glass and imaged with a EVOS FL 2

HeLa cells mounted with SlowFade Glass mountantHeLa cells expressing CellLight Mitochondria-GFP (Cat. No. C10600) were fixed and permeabilized. Tubulin was probed with Mouse Anti-beta-3 Tubulin Antibody (Cat. No. 322600) and Alexa Fluor 555 Goat Anti-Mouse Secondary Antibody (Cat. No. A21424). Actin was stained with Alexa Fluor 647 Phalloidin (Cat. No. A22287) and nuclei labeled with NucBlue Fixed Cell ReadyProbes Reagent (Cat. No. R37606). Cells were mounted in SlowFade Glass Antifade Mounting Medium (Cat. No. P36980) and imaged on an EVOS FL Auto2 microscope using a 20X objective.\" data-omni-action=\"View figure\">

HeLa cells mounted with SlowFade Glass mountant

Multiple cell lines stained with Alexa Fluor Plus 647 PhalloidinAlexa Fluor Plus 647 Phalloidin (Cat. No. A30107) staining provides superior signal/background as shown in multiple cells lines than Alexa Fluor 647 Phalloidin (Cat. No. A22287). Quantitative data acquisition and analysis was performed using the CellInsight CX5 High Content Screening (HCS) platform (Cat. No. CX51110). Images are exposure time and gain matched and Error bars represent standard deviation.\" data-omni-action=\"View figure\">

Multiple cell lines stained with Alexa Fluor Plus 647 Phalloidin

Multiple cell lines stained with Alexa Fluor Plus 647 PhalloidinAlexa Fluor Plus 647 Phalloidin (Cat. No. A30107) staining provides superior signal/background as shown in multiple cells lines than Alexa Fluor 647 Phalloidin (Cat. No. A22287). Quantitative data acquisition and analysis was performed using the CellInsight CX5 High Content Screening (HCS) platform (Cat. No. CX51110). Images are exposure time and gain matched\" data-omni-action=\"View figure\">

Multiple cell lines stained with Alexa Fluor Plus 647 Phalloidin

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